Recombinant Human SHH 重组人SHH

Description :
FAQ COA 已发表文献 Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. The Hedgehog (Hh) family of secreted proteins plays an essential role in metazoan development, and defects in the Hh pathway lead to congenital abnormalities such as holoprosencephaly and certain forms of cancer. Shhis the most widely expressed of the many ligands, and much of what is known about vertebrate Hh signaling stems from studies on Shh. Though similar in many ways to theDrosophilaHh pathway, several features of vertebrate Hh signaling make it unique. Product Properties Synonyms HHG1, HHG-1, HLP3, HPE3, MCOPCB5 Accession Q15465 GeneID 6469 Source E.coli-derived human Shh protein, Cly25-Gly197, with an N-terminal Met. Molecular Weight Approximately 19.8 kDa. AA Sequence IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 98% by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 g/mL, corresponding to a specific activity of > 1.0 103IU/mg.Fully biologically active when compared to standard. Endotoxin EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.