Recombinant Human PDGF-BB Protein 重组人血小板衍生生长因子BB

Description :
FAQ COA 已发表文献 Product Description Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma.In vitro, PDGF-BB stimulated MAP kinase activity and cell motility of isolated lymphatic endothelial cells. In vivo, PDGF-BB potently induced growth of lymphatic vessels. Expression of PDGF-BB in murine fibrosarcoma cells induced tumor lymphangiogenesis, leading to enhanced metastasis in lymph nodes.The PDGF signaling system contributes to and vascular remodeling.Growth factors such asPDGF exert potent effects on wound healing including the regeneration of tooth-supporting structures. Product Properties Synonyms Becaplermin, ODGF, PDGF-2,PDGFBB,PDGF-BB Accession P01127 GeneID 5155 Source E.coli-derived human PDGF-BBprotein, Ser82-Thr190, with an N-terminal Met. Molecular Weight Approximately 24.8kDa AA Sequence MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97 % by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 3 ng/ml, corresponding to a specific activity of > 3.3 105IU/mg. Endotoxin EU per 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution inPBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20 C. Further dilutions should be made in appropriate buffered solutions.