Recombinant Human PDGF-AA Protein

Description :
FAQ COA 已发表文献 PDGF is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The A-chain homodimers of the platelet-derived growth factor (PDGF AA) are widely expressed in normal and transformed cells. The mitogenic properties of PDGF AA are well established. FGF-2 and platelet-derived growth factor-A (PDGF-AA) are potent modulators of oligodendrocytes, the main responsible cells for myelin regeneration. Product Properties Synonyms PDGF1,PDGF-A,PDGFAA,PDGF-AA Accession P04085 GeneID 5154 Source E.coli-derived humanPDGF-AA, Ser87-His211, with an N-terminal Met. Molecular Weight Approximately 28.8kDa. AA Sequence MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >95% by SDS-PAGE analyses. Biological Activity The ED50for this effect is 50-_x005f_x001f_200 ng/mL in a fluorometric assay using the redox sensitive dye. Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Endotoxin 0.1EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in20 mM PB, 300 mM NaCl, pH 6.0. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.