Recombinant Human IL-3 Protein

Description :
FAQ COA 已发表文献 Product Description IL-3, also known as Interleukin-3, is a cytokine that is produced by activated T cells and mast cells. It plays a crucial role in regulating the growth, differentiation, and function of various cell types in the immune system.human IL-3 is an important cytokine that regulates the growth, differentiation, and function of immune cells. Its study contributes to our understanding of immune system regulation and its potential applications in immunotherapy and disease treatment. Product Properties Synonyms Interleukin-3, IL-3, Hematopoietic growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor Accession P08700 GeneID 3562 Source E.coli-derived human IL-3 protein, Ala20-Phe152. Molecular Weight Approximately 15.0 kDa. AA Sequence APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 96% by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 107IU/mg.Fully biologically active when compared to standard. Endotoxin EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.