Recombinant Human IFN-gamma Protein重组人干扰素γ

Description :
FAQ COA 已发表文献 Interferon-gamma(IFN-), also known as Type II interferon or immune interferon,is a pleiotropic cytokine which is now recognised as an important modulator of the immune response. Mature human IFN- exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits. It shares 90%amino acid (aa) sequence identity with rhesus IFN-, 59%_x005f_x001e_64%with bovine, canine, equine, feline, and porcine IFN-, and 37%_x005f_x001e_43%with cotton rat, mouse, and rat IFN-.Interferons have had increasing clinical usage in the treatment of a variety of disorders, at times being used in combination with chemo or radiotherapy.IFN- increases the potential immunogenicity of vascular endothelial cells by up-regulation of intercellular adhesion molecule-1 (ICAM-1) and class I MHC antigen expression and by induction of class II MHC antigens and certain chemokines. Product Specifications Synonyms Interferon-gamma,IFN-,IFG,IFI,IFNG,IFNgamma,IFN-gamma. Accession P01579 GeneID 3458 Source E.coli-derived human IFN-protein, Gln24-Gln166 with an N-terminal Met. Molecular Weight Approximately 16.9kDa. AA Sequence MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRGR RASQ Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 98% by SDS-PAGE and HPLC analyses. Biological Activity The ED50as measured in anti-viral assays using human HeLa cells infected with encephalomyocarditis (EMC) virus is 0.15-0.80 ng/ml.Fully biologically active when compared to standard. Endotoxin EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.