Recombinant Cynomolgus PDGFR beta/CD140b Protein,His Tag

Description :
Synonyms JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; CD140B; PDGFR-2; PDGFR-; PDGF R Uniprot No. P09619 Source Recombinant Human PDGF R beta/CD140b Protein is expressed from HEK293 with hFci tag at the C-terminal. Met1-Lys530. Molecular Weight The reduced monomer has a predicted molecular mass of 83.2 kDa. The apparent molecular mass of the protein is approximately 117 kDa in SDS-PAGE under reducing conditions. Purity > 90% as determined by SDS-PAGE and HPLC. Activity 1.Measured by its binding ability in a functional ELISA. 2.Immobilized mouse PDGF-B at 10g/mL (100L/well) can bind rat PDGFRB-Fc, the EC50of rat PDGFRB-Fc is 0.02-0.2 g/mL. Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Supplied as 0.22m filtered solution in PBS (pH 7.4).Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.