Recombinant Canine PD-1/PDCD1 Protein,hFc Tag

Description :
Synonyms PDCD1; PD1; CD279; SLEB2; PD-1 Source Canine PD-1/PDCD1 Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Leu25-Val170.[Accession | NP_001301026.1] Molecular Weight The protein has a predicted MW of 43.00 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Canine PD-1, hFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human PD-L1, mFc Tag with the EC50 of 30.7ng/ml determined by ELISA . Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.