Recombinant Human Siglec-10 (R119A) Protein,His-Avi Tag

Description :
Synonyms SLG2; SIGLEC10; MGC126774; PRO940 Source Human Siglec-10(R119A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Met17-Thr546.[Accession | Q96LC7-1] Molecular Weight The protein has a predicted MW of 62.64 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per ug by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human Siglec-10 (R119A) , His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-Siglec-10 (R119A) Antibody, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 20mM Tris,150mM NaCl, 200mM Arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.