Recombinant PE-Labeled Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein,His-Avi Tag

Description :
Synonyms MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD Source PE-Labeled Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-Labeled Human KRAS G12V (HLA-A*03:01) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin. It contains Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and VVVGAVGVGK peptide.[Accession | NP_002107.3(HLA-A*03:01)&P61769(B2M)&VVVGAVGVGK] 内毒素Endotoxin Less than 1EU per g by the LAL method. Activity ELISA Data: Immobilized HLA-A*11:01&B2M&KRAS G12D TCR at 1g/ml (100l/well) on the plate. Dose response curve for PE Labeled Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer, His Tag with the EC50 of 0.11g/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS, 0.2% BSA (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.