Recombinant Rhesus macaque IL-18 R1/CD218a Protein,hFc Tag

Description :
Synonyms CD218a; IL18Ra; IL18R1; IL1RRP; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL-18Ra Source Rhesus macaque IL-18 R1/CD218a Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Cys22-Gly329.[Accession | F7H8F9] Molecular Weight The protein has a predicted MW of 61.96 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Rhesus macaque IL-18 R1, hFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 0.42g/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.