Recombinant Human BTN3A1/CD277 Protein

Description :
Synonyms BTF5;BTN3.1;BTN3A1;CD277 Source Human BTN3A1/CD277 Protein is expressed from HEK293 without tag. It contains Gln30-Gly254.[Accession | O00481-1] Molecular Weight The protein has a predicted MW of 24.15 kDa. Due to glycosylation, the protein migrates to 26-32 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human BTN3A1, No Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.