Recombinant Human HGFR/c-MET Protein,hFc Tag

Description :
Synonyms MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor Uniprot No. P08581-1 Source Recombinant Human HGF R/c-MET Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Glu25-Thr932. Molecular Weight The mature form of HGF R is a heterodimer which can be cleaved into and chain. The protein has a predicted MW of 32.5 kDa ( chain) and 95.9 kDa ( chain Fc chimera). Due to glycosylation, the protein migrates to 45-55 kDa and 100-120 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGE . Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.