Recombinant Human IL-1R2/IL-1 RII/CD121b Protein

Description :
Synonyms IL-1RT2;IL-1R-beta;IL-1RT-2;IL-1R-2 Source Human IL-1R2/IL-1 RII/CD121b Protein is expressed from HEK293 without tag. It contains Phe14-Glu343.[Accession | NP_004624.1] Molecular Weight The protein has a predicted MW of 37.78 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human IL-1R2, No Tag at 2g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 0.21g/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.