Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein,His-Flag Tag

Description :
Synonyms CD257;TNFSF20;BLyS;BAFF Source Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus. It contains Thr141-Leu285.[Accession | Q9Y275-1] Molecular Weight The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human BAFF Trimer, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA .ELISA Data: Immobilized Human BAFF Trimer, His Tag at 1g/ml (100l/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 37.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.