Recombinant Human IL-18 R1/CD218a Protein,hFc Tag

Description :
Synonyms IL18R1;IL-1 R5;IL18Ra;CD218a Source Human IL-18 R1/CD218a Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Ala19-Arg329.[Accession | AAH69575.1] Molecular Weight The protein has a predicted MW of 62.52 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data:Human IL-18 R1, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-18, No Tag with an affinity constant of 0.73 nM as determined in SPR assay . Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.