Recombinant Human TNFR2/CD120b/TNFRSF1B Protein,hFc Tag

Description :
Synonyms CD120b; Etanercept; p75TBPII; p75TNFR; TNF RII; TNF-R2; TNF-R75; TNFR80; TNFRSF1B; TNFR2; TBPII; TNFBR; TNFR1B Source Human TNFR2/CD120b/TNFRSF1B Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Leu23-Asp257.[Accession | P20333-1] Molecular Weight The protein has a predicted MW of 51.89 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human TNF alpha, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA .ELISA Data: Immobilized Human TNFR2, hFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Anti-TNFR2 Antibody, hFc Tag with the EC50 of 19ng/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.