Recombinant Human CD47 Protein

Description :
Synonyms CD47;OA3;CD47 molecule;CD47 glycoprotein Source Human CD47 Protein is expressed from HEK293 without tag. It contains Gln19-Pro139.[Accession | Q08722-1] Molecular Weight The protein has a predicted MW of 13.72 kDa. Due to glycosylation, the protein migrates to 35-48 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human CD47, No Tag at 5g/ml (100l/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.24g/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.