Recombinant Biotinylated Human IL-3 Protein,His-Avi Tag

Description :
Synonyms IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF Source Biotinylated Human IL-3 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus. It contains Ala20-Phe152.[Accession | P08700] Molecular Weight The protein has a predicted MW of 16.9 kDa same as SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Biotinylated Human IL-3, His Tag at 1g/ml (100l/Well) on streptavidin (5g/ml) precoated plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 24.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.