Recombinant Mouse tPAProtein,hFc Tag

Description :
Synonyms t-PA; tPA; Alteplase; Reteplase; PLAT; Source Mouse tPAProtein is expressed from HEK293 with hFc tag at the N-terminus. It contains Ser33-Gln559, which consists of two chains: chain A (Ser36-Arg308) and chain B (Ile309-Gln559).[Accession | P11214] Molecular Weight The protein has a predicted MW of 85.00 kDa. The protein is activated by thermolysin and cleaved to a 2-chain protease, it migrates to 35-40 kDa (Chain A) and 30-35 kDa (Chain B) based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.