Recombinant Biotinylated Human CEACAM-5/CD66e Protein,His-Avi Tag

Description :
Synonyms CEACAM-5; CD66e; CEA; Meconium antigen 100; Carcinoembryonic; CEACD66e; DKFZp781M2392 Source Biotinylated Human CEACAM-5/CD66e Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Lys35-Ala685.[Accession | P06731-1] Molecular Weight The protein has a predicted MW of 74.23 kDa. Due to glycosylation, the protein migrates to 110-150 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Biotinylated Human CEACAM-5, His Tag at 2g/ml (100l/well) on the streptavidin precoated plate (5g/ml). Dose response curve for Anti-CEACAM Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.