Recombinant Human RNF43 Protein,His Tag

Description :
Synonyms E3 ubiquitin-protein ligase RNF43;RING finger protein 43;RING-type E3 ubiquitin transferase RNF43;RNF43 Source Human RNF43 Protein is expressed from HEK293 with His tag at the C-terminus. It contains Gly24-Tyr197.[Accession | Q68DV7-1] Molecular Weight The protein has a predicted MW of 20.14 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data:Human R-Spondin 3, His Tag immobilized on CM5 Chip can bind Human RNF43, His Tag with an affinity constant of 0.255 M as determined in SPR assay . Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.