Recombinant Canine EGFProtein,His Tag

Description :
Synonyms HOMG4; URG Source Canine EGFProtein is expressed from E.coli with His tag at the N-terminus. It contains Asn973-Arg1024.[Accession | Q9BEA0] Molecular Weight The protein has a predicted MW of 13.61 kDa. The protein migrates to 16-22 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Canine EGF, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.