Recombinant Human DKK1 C terminal Domain Protein,mFc Tag

Description :
Synonyms Dkk-1;hDkk-1;Dickkopf-1;Dickkopf-related 1 Source Human DKK1 C terminal Domain Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus. It contains Met178-His266.[Accession | O94907] Molecular Weight The protein has a predicted MW of 35.78 kDa. Due to glycosylation, the protein migrates to 45-53 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human DKK1 C terminal Domain, mFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.