Recombinant Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer Protein,His-Avi Tag

Description :
Synonyms Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; AFP; HPAFP; AFPD; FETA; MHC Source Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&FMNKFIYEI] Molecular Weight The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag at 2g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, His Tag with the EC50 of 0.31g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.