Recombinant Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer Protein,His-Avi Tag

Description :
Synonyms K-Ras 2;C-K-RAS;KRAS;MHC Source Human KRAS G12D(HLA-A*11:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M) and VVVGADGVGK peptide.[Accession | AAV53343.1(HLA-A*11:01)&P61769(B2M)&VVVGADGVGK] Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR at 1g/ml (100l/well) on the plate. Dose response curve for Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer, His Tag with the EC50 of 51.5ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.