Recombinant Cynomolgus IL-6 Protein,His Tag

Description :
Synonyms IFN-beta-2; IL6; IL-6; BSF-2; CDF; MGI-2A; Interleukin-6; HSF; IFNB2; HGF;IFN–2 Source Cynomolgus IL-6 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala28-Met212.[Accession | P79341] Molecular Weight The protein has a predicted MW of 22.06 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Cynomolgus IL-6, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.