Recombinant Biotinylated Human CA125/MUC16 Protein,His-Avi Tag

Description :
Synonyms CA125; CA-125; CA125MUC-16; FLJ14303; MUC16 Source Biotinylated Human CA125/MUC16 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Asp12783-Ser13467.[Accession | Q8WXI7] Molecular Weight The protein has a predicted MW of 77.98 kDa. Due to glycosylation, the protein migrates to 90-140 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human MSLN, hFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 16.5ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.