Recombinant Cynomolgus IL-12 Protein,His Tag

Description :
Synonyms CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12 Source Cynomolgus IL-12 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ile23-Ser328 (IL-12B) & Arg57-Ser253 (IL-12A).[Accession | G7P6S2(IL-12B)&XP_005546300.2(IL-12A)] Molecular Weight The protein has a predicted MW of 34.76 kDa (IL-12B) and 23.64 kDa (IL-12A). Due to glycosylation, the protein migrates to 45-48 kDa (IL-12B) and 38-44 kDa (IL-12A) based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Cynomolgus IL-12, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.