Recombinant Mouse HGF R/c-MET Protein,His Tag

Description :
Synonyms MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor Source Mouse HGF R/c-MET Protein is expressed from HEK293 with His tag at the C-terminus. It contains Glu25-Ala931.[Accession | P16056] Molecular Weight The mature form of HGF R is a heterodimer which can be cleaved into and chain. The protein has a predicted MW of 32.1 kDa ( chain) and 69.8 kDa ( chain). Due to glycosylation, the protein migrates to 40-50 kDa ( subunit) and 78-98 kDa ( subunit) based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Mouse HGF R, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.