Recombinant Biotinylated Human FcRn Protein,His-Avi Tag

Description :
Synonyms FcRn alpha chain; FCGRT; FcRn; FCGRT&B2M Source Biotinylated Human FcRn Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala24-Ser297(FCGRT)&Ile21-Met119(B2M).[Accession | P55899(FCGRT)&P61769((B2M)] Molecular Weight The protein has a predicted MW of 46.5 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data:Biotinylated Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 0.557 uM asdetermined in SPR assay . Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.