Recombinant Human DKK1 Protein,hFc Tag

Description :
Synonyms SK;Dkk1;Dickkopf-1 Source Human DKK1 Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Thr32-His266.[Accession | O94907] Molecular Weight The protein has a predicted MW of 52.54 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human DKK1, hFc Tag at 5g/ml (100l/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 1.69g/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.