Recombinant Biotinylated Human CD300A Protein,Avi Tag

Description :
Synonyms IGSF12; IRp60; CLM-8; IRC1; CD300a; CLM8; LMIR1; MAIR-I; Mcipr1; NKRL; Pigr4 Source Biotinylated Human CD300A Protein is expressed from HEK293 with Avi tag at the C-terminus. It contains Leu18-Pro180.[Accession | Q9UGN4-1] Molecular Weight The protein has a predicted MW of 19.46 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Biotinylated Human CD300A, Avi Tag at 0.5g/ml (100l/well) on the streptavidin precoated plate (5g/ml). Dose response curve for Anti-CD300A Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.