Recombinant Mouse APRIL/TNFSF13 Protein,His-Flag Tag

Description :
Synonyms CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2 Source Mouse APRIL/TNFSF13 Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus. It contains Ala96-Leu241.[Accession | Q9D777] Molecular Weight The protein has a predicted MW of 18.53 kDa. Due to glycosylation, the protein migrates to 24-30 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 90% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Mouse APRIL, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.