Recombinant Rat PDGF R beta/CD140b Protein,His Tag

Description :
Synonyms JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; CD140B; PDGFR-2; PDGFR-; PDGF R Source Rat PDGF R beta/CD140b Protein is expressed from HEK293 with His tag at the C-terminus. It contains Leu32-Val531.[Accession | Q05030] Molecular Weight The protein has a predicted MW of 57.36 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data:Human PDGFB, His Tag immobilized on CM5 Chip can bind Rat PDGF R beta, His Tag with an affinity constant of 67.56 nM as determined in SPR assay . Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.