Recombinant Mouse IGF1R/CD221 Protein,His Tag

Description :
Synonyms CD221; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGFR; JTK13; MGC142170; MGC142172; MGC18216 Source Mouse IGF1R/CD221 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu31-His936.[Accession | Q60751] Molecular Weight The protein has a predicted MW of 80.72 kDa (alpha subunit) and 23.0 kDa (beta subunit). Due to furin cleavage and glycosylation, the protein migrates to 110-140 kDa(alpha subunit) and 50-65 kDa(beta subunit) based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Mouse IGF1R, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human IGFI, hFc Tag with the EC50 of 2.3g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.