Recombinant Human MICA alpha 3 Protein,mFc Tag

Description :
Synonyms MICA alpha 3;MIC-A;MICA Source Human MICA alpha 3 Protein is expressed from HEK293 with mFc (IgG1) tag at the N-Terminus. It contains Arg105-Ser200.[Accession | NP_001276081.1] Molecular Weight The protein has a predicted MW of 36.8 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human MICA alpha 3, mFc Tag at 2g/ml (100l/well) on the plate. Dose response curve for Anti-MICA Antibody, hFc Tag with the EC50 of 76.9ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.