Recombinant Human PD-1/PDCD1 Protein,mFc Tag

Description :
Synonyms CD279; PD1;PDCD1; PD-1 Source Human PD-1/PDCD1 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus. It contains Leu25-Gln167.[Accession | Q15116-1] Molecular Weight The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 64-68 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human PD-1, mFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.17g/ml determined by ELISA .Immobilized Human PD-1, mFc Tag at 0.5g/ml (100l/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.