Recombinant Cynomolgus HGF R/c-MET Protein,His Tag

Description :
Synonyms HGF R; oncogene MET;MET; AUTS9 Source Cynomolgus HGF R/c-MET Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu25-Asn930.[Accession | A0A2K5UM36] Molecular Weight The mature form of HGF R is a heterodimer which can be cleaved into and chain. The protein has a predicted MW of 32.5 kDa ( chain) and 69.9 kDa ( chain). Due to glycosylation, the protein migrates to 45-52 kDa ( chain) and 75-82 kDa ( chain) based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Cynomolgus HGF R, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 38.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.2). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.