Recombinant Human TIGIT Protein,hFc Tag

Description :
Synonyms VSTM3; VSIG9;TIGIT; Source Human TIGIT Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Met22-Pro141.[Accession | Q495A1-1] Molecular Weight The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 48-50 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human TIGIT, hFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 20.9ng/ml determined by ELISA .Immobilized Biotinylated Human Nectin-2, His Tag at 1g/ml (100l/Well) on the streptavidin precoated plate (5g/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.22g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.