Recombinant Human PD-L1/B7-H1 Protein,mFc Tag

Description :
Synonyms PD-L1; PDL1;CD274;B7-H Source Human PD-L1/B7-H1 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus. It contains Phe19-Arg238.[Accession | Q9NZQ7-1] Molecular Weight The protein has a predicted MW of 50.9 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human PD-L1, mFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA .Immobilized Human PD-L1, mFc Tag at 2g/ml (100l/Well) on the plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 0.13g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.