Recombinant Human HGF R/c-MET Protein,His-Avi Tag

Description :
Synonyms HGF R; oncogene MET;MET; AUTS9 Source Human HGF R/c-MET Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu25-Thr932.[Accession | P08581-1] Molecular Weight The mature form of HGF R is a heterodimer which can be cleaved into and chain. The protein has a predicted MW of 32.5 kDa ( chain) and 72.1 kDa ( chain). Due to glycosylation, the protein migrates to 45-60 kDa ( subunit) and 80-100 kDa ( subunit) based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human HGF R, His Tag at 5g/ml (100l/Well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA .Immobilized Human HGF R, His Tag at 2g/ml (100l/Well). Dose response curve for Human HGF, hFc Tag with the EC50 of 0.16g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.