Recombinant Human HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein,His-Avi Tag

Description :
Synonyms b2 microglobulin; sHLA-G;HLA G antigen; HLAG Source Human HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide.[Accession | P17693-1(HLA-G)&P61769(B2M)&RIIPRHLQL] Molecular Weight The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Anti-HLA-G Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.