Recombinant Human ROBO4 Protein,His Tag

Description :
Synonyms FLJ20798; MRB;ECSM4; MGC133353 Source Human ROBO4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala27-Glu469.[Accession | Q8WZ75-1] Molecular Weight The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human ROBO4, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.