Recombinant Human Notch 3 Protein,His Tag

Description :
Synonyms Notch-3; Notch homolog 3;CADASIL; Source Human Notch 3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala1378-Ser1640.[Accession | Q9UM47] Molecular Weight The protein has a predicted MW of 30.18 kDa. Due to furin-like protease cleavage and glycosylation, the protein migrates to 27-30 kDa and 10-12 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human Notch 3, His Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.