Recombinant Cynomolgus GPC3/Glypican 3 Protein,His Tag

Description :
Synonyms Glypican 3; DGSX;GPC3; MXR7 Source Cynomolgus GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln25-His559.[Accession | XP_005594665.1] Molecular Weight The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 42 kDa, 70-135 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Cynomolgus GPC3, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 35.0ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.