Recombinant Human PRLR Protein,His Tag

Description :
Synonyms MFAB; HPRL;PRL-R; Source Human PRLR Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln25-Asp234.[Accession | P16471-1] Molecular Weight The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human PRLR, His Tag at 2g/ml (100l/Well) on the plate. Dose response curve for Anti-PRLR Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA . Formulation Lyophilized from 0.22m filtered solution in 50mM Tris, 150nM Nacl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.