Recombinant Biotinylated Human MICA Protein,His-Avi Tag

Description :
Synonyms MICA; MGC111087;FLJ60820; DAMA-345G11.2 Source Biotinylated Human MICA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu24-Gln308.[Accession | Q96QC4] Molecular Weight The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Anti-MICA Antibody, hFc Tag at 5g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 1.7g/ml determined by ELISA .Immobilized Human NKG2D, hFc Tag at 2g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 0.32g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.