Recombinant Human BTN3A1/CD277 Protein,hFc Tag

Description :
Synonyms BTF5; BTN3A1;CD277; BTN3.1 Source Human BTN3A1/CD277 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln30-Gly254.[Accession | O00481-1] Molecular Weight The protein has a predicted MW of 50.9 kDa. Due to glycosylation, the protein migrates to 58-63 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human BTN3A1, hFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.