Recombinant Biotinylated Moues GDF15 Protein (Primary Amine Labeling),hFc Tag

Description :
Synonyms NAG-1; MIC-1;GDF-15; PLAB Source Biotinylated Mouse GDF15 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-Terminus. It contains Ser189-Ala303.[Accession | Q9Z0J7] Molecular Weight The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Mouse GFRAL, His Tag at 2g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Mouse GDF15, hFc Tag with the EC50 of 10ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.