Recombinant Human TGF-beta RII/TGFBR2 Protein,mFc-Avi Tag

Description :
Synonyms TbetaR-II; TGFBR2;TGFR2; TbetaR-II Source Human TGF-beta RII/TGFBR2 Protein is expressed from HEK293 with mFc (IgG1) tag and Avi tag at C-Terminus. It contains Ile24-Asp159.[Accession | P37173-1] Molecular Weight The protein has a predicted MW of 44 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human TGF-beta RII, mFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 3, Avi Tag with the EC50 of 6.4ng/ml determined by ELISA .Immobilized Human Mature TGF beta 1, No Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 8ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.